Search results for "x-ray of kidney calculi"

Direct nucleation of calcium oxalate dihydrate crystals onto the surface of living renal epithelial cells in culture

1998

Direct nucleation of calcium oxalate dihydrate crystals onto the surface of living renal epithelial cells in culture. Background. The interaction of the most common crystal in human urine, calcium oxalate dihydrate (COD), with the surface of monkey renal epithelial cells (BSC-1 line) was studied to identify initiating events in kidney stone formation. Methods. To determine if COD crystals could nucleate directly onto the apical cell surface, a novel technique utilizing vapor diffusion of oxalic acid was employed. Cells were grown to confluence in the inner four wells of 24-well plates. At the start of each experiment, diethyloxalate in water was placed into eight adjacent wells, and the pla…